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投稿时间：2018-08-27
投稿时间：2018-08-27
中文摘要: 以黑豆为原料，利用单因素试验和响应面试验优化黑豆花青素提取工艺条件并评价其体外抗氧化活性。试验结果表明，黑豆花青素的最佳提取条件为：乙醇浓度60%、温度60℃、提取3 h、料液比1∶15（g/mL），黑豆花青素得率为（1.101±0.101）mg/g，黑豆花青素对DPPH自由基、羟基自由基和铁离子螯合剂的清除率分别为61.96%、84.82%和93.09%。黑豆花青素具有较强的抗氧化活性，可以作为天然抗氧化剂进行开发。
Abstract:Glycine max was used as raw material to optimize the extraction process of anthocyanin by single factor test and response surface method.And its antioxidative activity in vitro was evaluate.The results showed that，the extraction process was as follows：ethanol concentration 60%，temperature 60℃，extraction time 3 h，ratio of material to liquid 1 ∶15（g/mL）.The yield of Glycine max anthocyanin was（1.101±0.101）mg/g.The scavenging rate of DPPH radical，hydroxyl radical and iron ion chelating agent were 61.96% ，84.82%and 93.09%，respectively.Glycine max anthocyanins have strong antioxidant activity and can be developed as natural antioxidants.
文章编号：201906017 中图分类号： 文献标志码：
基金项目:吉林省教育厅“十三五”科学技术项目（JJKH20190771KJ）；吉林工程技术师范学院大学生创新创业训练计划项目（201810204028）
| Author Name | Affiliation |
| CAO Bai-ying，ZHANG Ya-ting，SUN Rui-tong，GAO Xiu-e，ZHANG Rui | Food Engineering College，Jilin Engineering Normal University，Changchun 130052，Jilin，China |
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