Abstract:Purification condition of total flavonoids from citrus leaves by macroporous resin and its antioxidant ability in vitro was studied.Based on the single factor experiment，the response surface method was used to design and optimize，taking the adsorption rate as the index，the effects of 3 factors，such as the concentration of the sample solution，the pH value of the sample solution and the flow rate of the sample solution，on the adsorption effect were investigated.And the eluting agent and its dosage were also investigated.The ability to remove hydroxyl radicals and DPPH free radicals before and after purification of citrus leaves flavonoids was studied.The results showed that the D101 macroporous resin had better purification of total flavonoids from citrus leaves.The extraction of total flavonoids concentration 2 mg/mL，the desorption velocity 1 BV/h，the pH of the sample liquid was 2.5，other conditions are the total flavonoids of the sample liquid：resin volume 7.7∶1（mg/L），the ratio of detergent dosage to resin quality （mL/g）was 5.6∶1.After purification，the total flavone content from 4.8%to 72.8%.In the same flavonoid concentration，the antioxidant capacity in vitro was better than that before purification.Macroporous resin purification process has a good enrichment effect on flavonoids in citrus leaves，and the purified flavonoids were the main contributors to the antioxidant capacity in total flavonoids.