Abstract:Abstract：Based on the single-factor experiment，used the extruded genetically modified soybeans as raw material and the extraction rate of soybean oil as the index，used the quantitative research methods of digital PCR to study the effects to endogenous and exogenous gene in the process of oil extraction by aqueous enzymatic，furthermore，the degradation of endogenous and exogenous genes in transgenic soybeans was explored.The optimal conditions were determined as follows：the ratio of solid to liquid was 1 ∶6（g/mL），the amount of alkaline protease was 1.8%，the temperature was 35℃，the time was 4.0 h，and the pH was 9.0.Under these conditions，the endogenous genes of transgenic soybean in the aqueous phase obtained by the aqueous enzymatic method were accounted for 65.065 2%，the exogenous genes were accounted for 81.742 1%；the endogenous genes were accounted for 16.892 5%in the solid phase，and the exogenous genes were accounted for 11.284 2%；the endogenous genes were accounted for 0.000 2%in the oil phase，and the exogenous genes were accounted for 0.000 4%.