Abstract:A rapid and accurate chromatographic method for simultaneous determination of γ -aminobutyric acid and glutamic acid in mulberry leaves was developed.By studying the effect of material to liquid ratio，extraction temperature and time on the extraction rate，the optimum extraction conditions were as follows：under the condition of 80℃，the ratio of material to liquid was 5∶1（mg/mL），and it was extracted two times，and each time was 5 min.2，4-two nitrobenzene was used as the pre column derivatization reagent，Agilent TC-18 column，and sodium acetate buffer solution-acetonitrile（50%）as mobile phase，gradient elution.This method had a good linear relationship in the range of 0-0.05 mg/mL.The relative standard deviation value of γaminobutyric acid and glutamic acid was＜2%，and the average recovery rate was 88.024%and 114.64%respectively.The method could be used for the detection of the content of γ-aminobutyric acid and glutamic acid in mulberry leaves.This study provides theoretical and technical support for the development and utilization of mulberry leaf tea rich in γ-aminobutyric acid.