Abstract:The conditions of pyrraline formation and used Lys-Glu as the simulation system to explore the effect of different processing conditions on the formation of pyrraline was studied.The optimization of the formating conditions，containing heating time，temperature，pH and the ratio of raw material，was carried out using by the combined use of one-factor-at-a-time method and response surface analysis（RSA）with Box-Behnken experimental design，using the content of pyrraline produced as the evaluation index.The pyrraline content in the model was determined by high performance liquid chromatography （HPLC）.The results showed that Lys-Glu simulation system，where the concentration of Glu and Lys was 0.5 mol/L and 0.6 mol/L，respectively，and the pH of the Buffer solution was 7.4，the maximum of pyrraline at 4.69 μmol/L could be achieved under the reaction time of 50 min under 110℃.