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投稿时间:2018-07-20
投稿时间:2018-07-20
中文摘要: 探究细叶韭花不同溶剂提取物的抗氧化活性及亚硝化反应的抑制作用。对细叶韭花采用4种不同极性的溶剂(水、95%乙醇、乙酸乙酯、石油醚)进行提取,并对不同提取物的生物活性成分、抗氧化活性及抑制亚硝化作用进行研究。结果显示细叶韭花水提取物中的总糖含量(total sugar content,TSC)及还原糖含量(reducing sugar content,RSC)较高,分别为(50.32±1.23)、(30.33±0.05)mg/g,而 95%乙醇提取物中总酚含量(total phenolics content,TPC)和总黄酮含量(total flavonoids content,TFC)较高,分别为(11.80±1.09)、(12.65±0.85)mg/g;细叶韭花各提取物中水提取物清除DPPH自由基、ABTS+自由基以及总还原能力最强;在体外模拟人体胃液的条件下(pH 3.0,温度37℃),各提取物均能有效地清除亚硝酸盐及阻断亚硝胺合成,并随着浓度的增大及反应时间的延长呈上升趋势,其中水提取物对亚硝酸盐的清除能力和亚硝胺合成阻断能力均强于其他提取物。细叶韭花具有较好的抗氧化活性及抑制亚硝化作用,将为其在抗氧化剂研究及新型防癌抗癌药物研究等方面提供科学理论依据。
Abstract:Antioxidant and inhibitory effect on N-nitrosation of Allium tenuissimum flowers extracts were studied.Four various polar solvents(water,95%ethanol,ethyl acetate,petroleum ether)were used to extract compounds from Allium tenuissimum flowers.In addition,the content of bioactive compounds,antioxidant properties and inhibitory effect on N-nitrosation of extracts were investigated.The results showed that total sugar content(TSC),reducing sugar content(RSC)which were highest in water extract,were (50.32±1.23)and(30.33±0.05)mg/g,respectively.While total phenolics content(TPC),total flavonoids content(TFC)which were highest in 95%ethanol extract,were (11.80±1.09),(12.65±0.85)mg/g,respectively.The effects of scavenge free radical induding DPPH,ABTS+and total reducing power were best in water extract.In simulated gastric environment in vitro(pH 3.0,temperature 37℃),each extracts could effectively scavenge nitrite and inhibit N-nitrosames formation,and presented an increasing trend with the increase of concentration and reaction time,in which water extract showed that nitrite scavenging activity and inhibitory activity of N-nitrosamines formation were best.The results indicated that Allium tenuissimum flowers had good antioxidant activity and inhibitory effect on nitrosation,which would provide the scientific theory basis for antioxidants research and new anti-cancer drug research.
keywords: Allium tenuissimum flowers extracts bioactive compounds antioxidant inhibitory effect on nitrosation
文章编号:201902010 中图分类号: 文献标志码:
基金项目:人才事业发展项目(228545021)
Author Name | Affiliation |
LI Mei-ping,DING Peng-xia,LI Rong,ZHANG Sheng-wan,GUO Cai-xia | College of Life Science,Shanxi University,Taiyuan 030006,Shanxi,China |
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