Abstract:The anthocyanins were extracted from the roselle dried calyx with ethanol solvent.On the basis of single factor experiment of ethanol concentration，extraction temperature，material liquid ratio and extraction time，and a response surface optimization experiment with four factors and three levels was designed by Box-Behnken.The results showed that the most important factor affecting anthocyanin content was ethanol concentration，followed by extraction temperature and extraction time，and the smallest factor was the solidliquid ratio.Finally，the optimum extraction parameters were determined as follows：ethanol concentration was 38%，extraction temperature was 66.6℃，the solid-liquid ratio was 1∶24（g/mL），and extraction time was 91 min.Under these conditions，the anthocyanin content from roselle was 5.285mg/g.