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食品研究与开发:2019,40(1):111-117
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无花果酶解制汁工艺优化及抗氧化活性
(1.江苏省农业科学院农产品加工研究所,江苏南京210014;2.苏州西山国家现代农业示范园区有限责任公司,江苏苏州215111;3.东北农业大学园艺园林学院,黑龙江哈尔滨150030)
Optimization of Enzymatic Hydrolysis for Production of Fig Juice and Antioxidant Activity
(1.Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;2.Suzhou Xishan National Modern Agriculture Demonstration Zones Limited Liability Company,Suzhou 215111,Jiangsu,China;3.Horticultural Garden College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)
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投稿时间:2018-06-26    
中文摘要: 在单因素试验基础上采用响应面法优化纤维素酶和果胶酶制备无花果汁的工艺参数,并通过测定果汁的DPPH自由基清除率、抑制羟自由基活力及总抗氧化能力,评价其体外抗氧化活性。结果表明,无花果最佳酶解制汁工艺参数为纤维素酶添加量2.5%、果胶酶添加量0.6%、酶解温度50℃、酶解时间90 min,此条件下无花果出汁率为75.13%,汁中多酚含量为626.79 μg/mL;所制备无花果汁具有较高的抗氧化活性。研究结果可为无花果汁的产业化生产提供理论依据。
中文关键词: 无花果  纤维素酶  果胶酶  响应面  多酚  抗氧化
Abstract:The response surface methodology was used to optimize the technological parameters for the preparation of fig juice by cellulase and pectinase.The scavenging rate of DPPHfree radical,inhibition of hydroxyl radical activity and total antioxidant activity of the juice were determined to evaluate its antioxidant activity in vitro.The results showed that the optimum processing parameters were as follows:cellulase amount 2.5%,pectinase amount 0.6%,enzymolysis temperature 50℃,enzymolysis time 90 min.On this condition,the juice yield of figs was 75.13%,and the polyphenol content in the juice was 626.79 μg/mL;the prepared fig juice had high antioxidant activity.The results can provide theoretical basis for the industrial production of fig juice.
文章编号:201901019     中图分类号:    文献标志码:
基金项目:苏州市农业科技创新项目(SNG201653);江苏省农业科学院院基金(6111682)
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