Abstract:Korean pine seed oil has much nutrients and many biological functions，poor bioavailability and low added value has become its the bottleneck of development.The Korean pine seeds were used as raw materials，and the oil extraction rate and the amount of pinolenic acid（PLA）was used as indicators.Disposed on ultrasonic conditions，single-factor hydrolysis of snail enzymes and its hydrolysis conditions were optimized by response surface methodology.The results showed that optimal extraction conditions were obtained as follows：under 3.00 h of hydrolysis using 2 364.00 U/g of alcalase at 51.00℃and pH 8.40 and then by 1.00 h of hydrolysis using 39.00 mg from 10 g pine milk of snail enzyme at 44.00℃and pH 7.00 with a substrate to water ratio of 1 ∶5（g/mL）.Under the conditions，the yield of pine nut oil and the yield of PLA were reached 93.87%and 855.77 mg from 10 g pine milk，respectively，which were higher than traditional method results significantly.Meanwhile，under the optimal conditions，the IC50value for free radical scavenging of Korean pine seed oil was 8.52 mg/mL，which was higher than perilla seed oil and other vegetable oils.