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投稿时间:2018-06-13
投稿时间:2018-06-13
中文摘要: 对10个产地北沙参中多糖的提取率、可溶性总糖含量、黏度、分子量分布、抗氧化及降血糖活性进行了研究。结果表明:产地不同,北沙参多糖的理化性质和生物活性存在差异,但具有相似的紫外、红外光谱学性质及分子量分布。多糖提取率范围在(13.20±0.07)%~(26.75±0.01)%之间,可溶性总糖含量范围为(81.28±0.02)%~(89.10±0.10)%,黏度范围为(6.55±0.05)Pa·s~(9.40±0.10)Pa·s;其中产地为河北安国的北沙参多糖对 DPPH 和羟基自由基的清除率以及对α-葡萄糖苷酶的抑制率最高,半数清除率浓度(half maximal inhibitory concentration,IC50)分别为0.237、0.564、10.614 mg/mL。
中文关键词: 北沙参 多糖 理化性质 抗氧化 α-葡萄糖苷酶抑制剂
Abstract:The extraction rate,soluble total sugar content,viscosity,molecular weight distribution,antioxidant and hypoglycemic activities of Glehnia littoralis polysaccharides from 10 habitats were studied.The results showed that polysaccharides from different origins had different physicochemical properties and biological activities,but they had similar ultraviolet and infrared spectroscopy properties and molecular weight distribution.The extraction rate of polysaccharides ranged from(13.20±0.07)%to(26.75±0.01)%,the total soluble sugar content ranged from(81.28±0.02)%to(89.10±0.10)%,and the viscosity ranged from(6.55±0.05)Pa·s to(9.40±0.10)Pa·s.The polysaccharides from the Glehnia littoralis of Hebei Anguo had the highest scavenging rate of DPPH and hydroxyl radicals and the highest inhibition rate of α-glucosidase,and the half maximal inhibitory concentration(IC50)was 0.237,0.564 mg/mL and 10.614 mg/mL,respectively.
keywords: Glehnia littoralis polysaccharide physicochemical properties antioxidation α-glucosidase inhibitor
文章编号:201824004 中图分类号: 文献标志码:
基金项目:第四次全国中药资源普查项目(Z135080000022);河北省自然科学基金项目(H2016208059、H2016208058、H2017423023);河北省高等学校科学技术研究项目(ZD2016009);河北省现代农业技术体系中药材产业创新团队建设(20170401458-2)
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