Abstract:It aimed to optimize polysaccharides extraction from Mangifera indica L.rind and investigate its antioxidant activity in vitro.The mathematical model was established by response surface method（RSM）based on the results of single factor experiments，using polysaccharides extraction rate as response value，and using the pH value，extraction time，cellulase addition amount and the ratio of water to material as experimental factors，which was used to screen optimum polysaccharide extraction conditions from Mangifera indica L.rind.Antioxidant activity in vitro of polysaccharides was studied by DPPH·and·OH elimination method.The optimum conditions obtained by RSM were as follows：the pH value was 5.0，extraction time duration was 100 min，the cellulase addition amount was 10.5 mg/mL，the ratio of water to feedstock was 7.6 ∶1（mL/g），extraction temperature was 45℃.Under the optimal conditions，there was a difference of less than 5%between predicted extraction rate 5.28%and experimental extraction rate 5.17%.The polysaccharide yield was most significantly affected by extraction time，followed by water to material ratio，cellulase addition amount，and pH value.IC50of DPPH·and·OH were 1.385 mg/mL and 3.612 mg/mL，respectively.Antioxidant activity in vitro of sample polysaccharides was weaker than those of vitamin C.