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食品研究与开发:2018,39(23):31-36
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紫玉米、笃斯越桔花色苷与蛋白结合作用比较
于情情,王丹,马越,王宇滨,张敏,赵煜炜,赵晓燕
((1.沈阳农业大学食品学院,辽宁沈阳110161;2.北京市农林科学院蔬菜研究中心,北京市果蔬农产品保鲜与加工重点实验室,农业部蔬菜产后处理重点实验室,北京100097;3.龙大食品集团有限公司,山东莱阳265231))
Bovine Serum Albumin-bingding Property Comparison of Purple Corn and Vaccinium uliginosum linn Anthocyanin
YU Qing-qing,WANG Dan,MA Yue,WANG Yu-bin,ZHANG Min,ZHAO Yu-wei,ZHAO Xiao-yan
((1.College of Food Science and Engineering,Shenyang Agricultural University,Shenyang 110161,Liaoning,China;2.Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science;Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing;Key Laboratory of Vegetable Postharvest Processing,Ministry of Agriculture,Beijing 100097,China;3.Longda Food Group Co.,Ltd.,Laiyang 265231,Shandong,China))
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投稿时间:2018-03-21    
中文摘要: 为了揭示紫玉米和笃斯越桔两种花色苷与牛血清白蛋白结合作用的不同,采用荧光光谱法、Zeta电位、结合粒径、胃肠吸收模拟等方法,对紫玉米、笃斯越桔花色苷与牛血清白蛋白的结合效果及对花色苷稳定性的影响进行研究。结果表明,与牛血清白蛋白结合后紫玉米及笃斯越桔两种花色苷均导致荧光强度降低,且随着花色苷浓度的增加而降低,证明两种花色苷均与牛血清白蛋白发生相互作用;紫玉米花色苷、笃斯越桔花色苷与牛血清白蛋白的结合摩尔比分别为16与24;两种花色苷均与牛血清白蛋白形成较稳定的10 nm左右粒径的颗粒;牛血清白蛋白提高了花色苷的胃肠吸收稳定性,紫玉米花色苷的稳定性提高效率高于笃斯越桔花色苷。
中文关键词: 紫玉米  花色苷  荧光光谱  Zeta电位
Abstract:The methods of fluorescence spectrometry,Zeta potential,particle size and simulated digestive system model in vitro were used to reveal the difference between the two anthocyanins of purple corn and Vaccinium uliginosum linn.In this study the properties of nanoparticles and the effect of bovine serum albumin(BSA) on the stability of purple corn anthocyanin and Vaccinium uliginosum linn anthocyanin were investigated.The results showed that the fluorescence intensity decreased with the increase of the concentration of the anthocyanin in the purple corn and Vaccinium uliginosum linn,indicating BSA had interacted with the anthocyanins from purple corn and Vaccinium uliginosum linn.The molar ratio of bovine serum albumin to anthocyanins of Vaccinium uliginosum linn and purple corn were 1 ∶16 and 1 ∶24;two kinds of anthocyanins with bovine serum albumin formed a stable particle size of anthocyanin-bound BSA(10 nm);bovine serum albumin improved the stability of anthocyanins in vitro gastrointestinal model system;compared with Vaccinium uliginosum linn anthocyanin,BSA bound purple corn anthocyanin exhibited a remarkable effect on the oxidation staility of anthocyanins.
文章编号:201823006     中图分类号:    文献标志码:
基金项目:北京市农林科学院青年科研基金(QNJJ201603);北京市农林科学院科技创新能力建设专项(KJCX20170205);现代农业产业技术体系建设专项资金资助(CARS-25)
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