Abstract:Abstract：To study the effects of garlic polysaccharide on the intestinal micrflora dysbiosis in acute alcoholinduced hepatic injury（AAHI）mice by using enterobacterial repetitive intergenic consensus-polymerase chain reaction（ERIC-PCR）and polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）techniques.AAHI model in mice was built by Erguotou.The diversity，similarity analyses and the sequences of the dominant bands were carried out by ERIC-PCR and PCR-DGGE techniques.ERIC-PCR analysis showed that the intestinal microflora structure was changed in AAHI group，the band of 360 bp was dominant band，and the intensity of band of 610 bp was decreased.In garlic polysaccharide-pretreated group，the intensity of band of 360 bp was decreased.PCR-DGGE analysis showed that Shannon-Weaver indexes decreased in AAHI group，and Eubacterium and Lactobacillus were both decreased obviously.In garlic polysaccharide-pretreated group，Shannon-Weaver indexes and evenness indexes were the highest，and Eubacterium and Lactobacillus were both increased.Garlic polysaccharide can improve the growths of Eubacterium and Lactobacillus，and regulate intestinal microflora dysbiosis associated with alcohol-induced hepatic injury.