Abstract:In order to reduce the environmental pollution and waste of by-products during the process of aquatic products processing and to increase the added value of aquatic products，the process of preparation collagen chelate calcium chelate of Lophius litulon bone was studied.Collagen peptide chelated calcium was prepared under the suitable conditions with the fish bones as raw materials，and the effects of temperature，mass ratio of fish collagen peptide to fish bone extract，time，pH on chelating rate and calcium content in chelate impact.According to the results of single factor test，the collagen peptide chelating calcium process was carried out three leves orthogonal experiment of four factors，and the optimum technological parameters of calcium chelation for collagen peptide were obtained at 30℃for 50 min.The ratio of calcium chelate to Ca2+and Ca2+in the chelating solution was 2 ∶1 and pH 6，respectively，and the content of calcium in the chelate was 98.00%and 21.29%，respectively.The chelated calcium amino acid composition analysis，in which，with a typical collagen polypeptide amino acid content of 17.37%of the total amino acid content.