Abstract:The research aimed to study extraction technology of total flavonoids from citrus peels and its hypoglycemic effect．Using citrus peels as raw material，the effects of different ethanol concentration，ratio of solid to liquid，extraction time and extraction times were taken to optimize extraction technology of total flavonoids from citrus peels by orthogonal design．The diabetic mouse model was established by the method of alloxan intraperitoneal injections．Blood glucose concentration of the mice were randomly divided into five groups：the group of metformin positive drug，the comparison group in the model mice with solvent，the blank comparison group in the normal mice，the groups of high，medium and low dose alcohol extracts.Using this method，the effect of total flavonoids of citrus peel on reducing blood sugar was observed.The best extract technology was to distill with 10 times 70%ethanol，solid-liquid ratio1 ∶20 （g/mL），refluxed 2 times and extracted for 2 h.The extraction temperature was 85℃.The extraction rate of total flavonoids was 7.68%.Animal experiments showed that：blood glucose concentration of each group in the model mice was increased．After lavaging with alcohol extracts for 14 d，blood glucose concentration was declined obviously.However，the blood glucose concentration of mice inthe blank group was not varied obviously.