Abstract:The influence of Wo of dodecyl-trimethyl ammonium chloride（DTAC）reverse micelles on protein extracted was investigated by fluorescence method.The results showed that with the water content of reverse micelles increased，the structure of protein extraction changed，and surface hydrophobicity increased.Emulsifying activity of protein declined slightly，emulsion stability increased significantly.There was a significant negative correlation between surface hydrophobicity and emulsifying activity，and a significant positive correlation between surface hydrophobicity and emulsifying stability.