Abstract:In recent years，the probiotic function of Lactobacillus has become the focus of research，the adhesion character of Lactobacillus to the intestinal mucosa is considered one of the important indicator for evaluating its probiotic function.New methods for studying the adherence character of Lactobacillus have also been emerging，such as surface plasmon resonance （SPR），computer molecular simulation（CMS）.Understanding the nature of Lactobacillus-mucus interactions could be the key to elucidating the mechanisms of Lactobacillus adhesion within the host.At the same time，the physicochemical property of Lactobacillus and the external environment also affect its adhesion ability，such as cell growth stage，concentration of bacterial，pH value，protease and so on.This paper reviewed the research progress about the study methods，mechanism and the influence factors of Lactobacillus adhesion，and the future trends for research were also prospected.