Abstract:56 strains of yeasts were isolated from the fermentation liquids of Ningxia melon.After screening and re-screening of WL nutrient medium，TTC chromogenic method，Durham tube method and sensory evaluation.1 yeast strain was selected for its prominence on strong ethanol-producing and high fermenting ability，fruity and flavor，typical strong，termed NT13．The tolerance capacity test showed that NT13 had the best performance，such as sugar，alcohol，SO2，pH.Alcohol yield could reach 10.9%vol by pycnometer method.About 2 hours later，the yeast concentration was logarithmic phase and stagnate phase was from 10 hours.NT13 was determined saccharomyces cerevisiae by testing its physiology and biochemistry，26S rDNA D1/D2 sequencing analysis.