Abstract:Spore germination was induced by 2，6-pyridinedicarboxylic acid （DPA），L-alanine and inosine，and the cortex-lytic enzyme was extracted from the germinated spores suspension.The cortex-lytic enzyme was analyzed by SDS-PAGE vertical plate electrophoresis after ultra-filtration.The results indicated that DPA，L-alanine，inosine and L-alanine combined with inosine all induced spore germination efficiently，while the spore germination induced with DPA was the best.During SDS-PAGE gel electrophoresis，the injection volume of 15 μL and 10%separation gel were better for proteins separation，and there were four protein bands on the gel plate.According to the standard curve of protein's molecular weight，the molecular weight of the target proteins were approximately 61.10，48.64，43.25 and 31.28 kDa，respectively.In this study，the cortex-lytic enzyme was extracted and preliminarily purified，which provided experimental materials for the subsequent study on the effect of high-pressure thermal sterilization（HPTS）on the activity and structure of the enzyme.It was helpful to elucidate the mechanism of spore inactivation under HPTS and promote the application of HPTS in food industry.