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投稿时间:2017-08-11
投稿时间:2017-08-11
中文摘要: 采用超滤法对巢脾多糖进行分级分离,获得分子量为 4 kDa~10 kDa(HCP-1)、10 kDa~50 kDa(HCP-2)、50 kDa~100 kDa(HCP-3),大于 100 kDa(HCP-4)4个不同分子量段的巢脾多糖,对 4个分子量段巢脾多糖的理化性质和抗氧化活性进行分析。结果显示巢脾多糖中蛋白质含量随着分子量的增大而增高,还原糖含量则随着分子量的增大而减小;各分子量段巢脾多糖紫外及红外光谱特征相似,而单糖组成及含量差异较大,HCP-3及HCP-4中单糖种类及含量相比于HCP-1及HCP-2都较为丰富。体外抗氧化活性分析结果显示,在一定浓度范围内,各分子量段多糖对超氧自由基、羟自由基清除能力及总抗氧化能力与多糖浓度呈明显的量效关系,其中HCP-4的抗氧化活性最强。
Abstract:The honeycomb polysaccharides (HCP)was separated to 4 different ranges of molecular weight by ultrafiltration membrane,which were 4 kDa-10 kDa(HCP-1),10 kDa-50 kDa(HCP-2),50 kDa-100 kDa(HCP-3)and >100 kDa (HCP-4),and the physicochemical properties and antioxidant activity of the 4 HCPs were analyzed.The results showed that the protein content in the HCPs increased with the increase of molecular weight,but the reducing sugar content decreased with the increase of molecular weight;UV absorption and infrared spectrum characteristics of the 4 HCPs were similar,however,the monosaccharide composition were greatly different,the kinds and content of monosaccharide in HCP-3 and HCP-4 were more abundant compared with HCP-1 and HCP-2.According to the results of analysis in the certain concentration range,there was a clear concentration-response relationship of HCPs concentrations and the antioxidative activity,including total antioxidant capacity,the scavenging capacity on the super oxygen free radical and hydroxyl radical.HCP-4 showed the strongest antioxidant activity of the 4 HCPs.
keywords: honeybee comb polysaccharide molecular weights physicochemical properties antioxidation activity
文章编号:201804001 中图分类号: 文献标志码:
基金项目:国家自然科学基金青年科学基金项目(31502020);江苏农牧科技职业技术学院重点科研课题(NSFRC1401);江苏农牧科技职业技术学院科研课题(NSFPT201521);校企合作课题(00010115004)
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