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食品研究与开发:2018,39(2):149-154
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水蒸气蒸馏与超临界提取来凤凤菊成分的HS-GC/MS分析
邢玉娟,陈新*,刘梁,史茹茹,彭彩暾
(武汉轻工大学生物与制药工程学院,湖北武汉430023)
HS-GC/MS Analysis of the Extracts by Steam Distillation and Supercritical-CO2Fluid Extraction from Dendranthelna Indicum
XING Yu-juan,CHEN Xin*,LIU Liang,SHI Ru-ru,PENG Cai-tun
(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China)
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投稿时间:2017-07-05    
中文摘要: 分析比较水蒸气蒸馏法与超临界CO2萃取法提取来凤凤菊化学成分的差异。通过水蒸气蒸馏提取和超临界CO2萃取法提取来凤凤菊中的化学成分,运用顶空气相色谱-质谱联用技术(headspace extraction-gas chromatography-mass spectrometry,HS-GC/MS)对其成分进行分离鉴定,用峰面积归一化法进行定量分析。水蒸气蒸馏提取法共分离出161种成分,鉴定出其中的56种,主要成分是:α-水芹烯(10.70%)、2,2,6-三甲基-6-乙烯基四氢-3-吡喃酮(7.14%)、芳樟醇(11.90%)、芳樟醇氧化物(7.95%)等;超临界CO2提取法共分离出121种成分,鉴定出其中的38种,主要成分是α-水芹烯(11.91%)、邻伞花烃(22.59%)、芳樟醇(6.95%)、芳樟醇氧化物(5.75%)等。两种不同的提取法共分离鉴定27种相同的化学成分,水蒸气蒸馏法多分离鉴定18种成分。
Abstract:To analyze the chemical constituent of the extracts from Dendranthelna indicum by different extraction methods.The compounds were extracted by steam distillation and supercritical-CO2fluid extraction.Constituents were separated and identified by headspace extraction-gas chromatography-mass spectrometry(HS-GC/MS),and were quantified by the accuracy of area percent.161 components were separated among which 56 were identified,the main components were alpha.-Phellandrene(10.70%),Tetrahydro-2,2,6-trimethyl-6-vinyl-3--pyranone(7.14%),Linalool(11.90%),Linalool Oxide(7.95%)by steam distillation.121 components were separated among which 38 were identified,the main components were alpha.-Phellandrene(11.91%),o-Cymene(22.59%),Linalool(6.95%),Linalool Oxide(5.75%)in the supercritical-CO2fluid extraction.27 chemical components of the extracts were the same by the two extraction methods,but there are more than 18 components in the steam distillation.
文章编号:201802026     中图分类号:    文献标志码:
基金项目:国家自然科学基金项目(31370369);国家自然科学基金项目(21602166)
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