Abstract:Tea Tree from Guangdong province was used as the raw materials to extract total flavonoids.A novel approach，ultrasonic-assisted method，for preparation of total flavonoids were developed.The optimal ultrasonic-assisted treatment was explored using response surface methodology.The results showed that the optimum extraction condition were ethanol concentration 60%，liquid ratio 1∶30（g/mL），extraction time 40 min.Under the optimization conditions，the experimental yield was 29.7 mg/g.The method is reasonable and feasible，can provide a theoretical reference for the development of new flavonoids in tea plant.