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食品研究与开发:2018,39(2):56-61
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响应面优化超声辅助提取豌豆总黄酮工艺研究
康永锋1,2,3,薛永刚4,*,韩巧英1
(1.上海海洋大学食品学院,上海201306;2.食品科学与工程国家级实验教学示范中心(上海海洋大学),上海201306;3.农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306;4.正宁县人民医院,甘肃庆阳745300)
Optimization of the Ultrasonic Extraction Technology of Flavonoids from Pisum sativum Linn Using Response Surface Analysis
KANG Yong-feng1,2,3,XUE Yong-gang4,*,HAN Qiao-ying1
(1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;2.National Experimental Teaching Demonstration Center for Food Science and Engineering(Shanghai Ocean University),Shanghai 201306,China;3.Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China;4.Zhengning County People's Hospital,Qingyang 745300,Gansu,China)
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投稿时间:2017-07-11    
中文摘要: 采用超声波辅助工艺提取豌豆总黄酮,响应曲面法对提取工艺进行优化。通过对乙醇含量、pH值、提取时间、超声波功率、液料比、温度6个工艺条件对豌豆总黄酮提取率影响的考察,选取了影响较大的温度、液料比、乙醇含量3个工艺条件进行响应面分析。使用响应面分析软件分析,得到的超声波辅助提取豌豆总黄酮的最佳工艺条件:温度61℃、液料比21∶1(mL/g)、乙醇含量65%、超声波功率500 W、pH=8、超声时间30 min。在此条件下得到的最佳提取率为黄酮1.101 mg/g。初步研究表明豌豆黄酮提取液具有一定抗氧化作用。
Abstract:The ultrasonic extracting technology of flavonoids from peas was optimized by response surface analysis.The process conditions of volume percentage of ethanol,pH value,extraction time,ultrasonic power,the ratio of fluid-solid and extraction temperature were studied in the single factor experiment.The process conditions of extraction temperature,the ratio of fluid-solid and volume percentage of ethanol were discussed in the response surface analysis.The result showed that the optimum process conditions were:61 ℃ of the extraction temperature,21∶1(mL/g)of the ratio of fluid-solid,65%of the volume percentage of ethanol.Under the optimum condition,the ultrasonic extraction yield of flavonoids was 1.101 mg/g.Beans isoflavone showed antioxidant activity in vitro.
文章编号:201802010     中图分类号:    文献标志码:
基金项目:上海市教委实验技术队伍建设项目(B1-5407-13-0000-7);上海海洋大学公共化学课程群教学团队(B1-5003-15-000112)
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