Abstract:A method has been developed to determine 53 kinds of plastic additives including antioxidant，plasticizer，ultraviolet light absorber and so on in highland barley wine by extraction-ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）.Samples were diluted to 20%alcohol with water，and extracted with acetonitrile and separated with salting out.The analytes were separated on a reversed phase ACQUITY BEH C18column （50 mm×2.1 mm，1.7μm）and eluted gradiently with methanol and 0.1%formic acid，and multiple reaction monitoring mode （MRM）was used with electrospray ionization in positive ion mode （ESI+）.Analytes were quantified using external standard method.The linear calibration curves were obtained in a wide range with the linear relative coefficients（r2）more than 0.99.Recoveries from 3 spiked levels ranged 71.8%-96.3%and the relative standard deviation（Relative Standard Deviation，RSDs）were 1.6%-14.7%（n=6）.The limits of detection （Detection Limit，LODs）and the limits of quantification（Quantification Limit，LOQs）for the analytes were 0.2μg/L-40μg/L and were 0.6μg/L-133μg/L，respectively.Twenty kinds of plastic additives are found in 12 highland barley wine samples.