Abstract:High performance liquid chromatography（HPLC）was used to qualitatively and quantitatively determine curcumin in millet and its uncertainty was analyzed.Acetonitrile-4%acetic acid（45+55）solution was used as the mobile phase，and 1.0 mL/min as the flow rate，and C18as the analysis column.Good linear relationship was obtained in the concentration range of 0.585 μg/mL-4.88 μg/mL of curumin，with correlation coefficient of 0.999 9.Under the optimized conditions，the average recoveries were 92%.The limit of detection（S/N=3）of curcumin was 0.08 mg/kg.The combined uncertaincy was 1.937%and the expanded uncertainty was X=（7.15±0.29）mg/kg；k=2.The method was sample，reproducible，low detection limit，and can be used to determine the illegal staining of curcumin in millet.