Abstract:In order to get more oligopeptides，establish low-value marine fish meal hydrolysis conditions，degree of hydrolysis as the indexes to compare the effect of hydrolysis of alkaline protease，neutral protease and so on the many kinds of protease.Alkaline protease and neutral protease was selected as the best protease.The hydrolysis conditions was optimized by response surface methodology.The results showed the optimal enzymatic hydrolysis conditions：the alkaline protease and neutral protease enzyme activity ratio of 6 ∶4 successively joined，enzyme amount was 2 500 U/g，the alkaline protease kept hydrolyzing for 2 h when the pH was 8.Until the pH value dropped to 7.0，the neutral protease was added and continued stirring for another 3 h，enzymolysis temperature 50℃.Under the condition of the hydrolysis degree as high as 41.84%.