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投稿时间：2017-04-12
投稿时间：2017-04-12
中文摘要: 建立一种高效液相色谱法（HPLC），可同时定量绿咖啡豆中绿原酸和咖啡碱的含量，并分析不同成熟度绿咖啡豆中绿原酸和咖啡碱含量变化。采用Agilent ZORBAX ODS-C18色谱柱，以0.5%乙酸水-乙腈为流动相，梯度洗脱，检测波长274 nm，流速1.0 mL/min，进样量10 μL，柱温35℃。结果显示，绿原酸和咖啡碱的浓度线性范围分别为5.0 mg/L～100.0 mg/L（r2=0.999 9）和 5.1 mg/L～101.6 mg/L（r2=0.999 9），检出限分别为 0.25 mg/L 和 0.05 mg/L，相对标准偏差分别为1.56%和1.30%，回收率范围分别为98.86%～101.11%（RSD=1.02%）和98.70%～101.19%（RSD=0.83%）。
中文关键词: 绿咖啡豆 绿原酸 咖啡碱 高效液相色谱法（HPLC） 含量
Abstract:A high performance liquid chromatography method was developed for simultaneous determination of chlorogenic acid and caffeine in raw coffee beans.The chlorogenic acid and caffeine contents in raw coffee beans of different maturity were analyzed.The separation was performed on a Agilent ZORBAX ODS-C18 column with a gradient elution system.The mobile phase consisted of 0.5%acetic acid solution and acetonitrile.The detection UV wavelength was at 274 nm.The flow rate was 1.0 mL/min.The injection volume was 10 μL.The column temperature was 35℃.The results showed that the linear ranges of chlorogenic acid and caffeine were 5.0 mg/L-100.0 mg/L （r2=0.999 9）and 5.1 mg/L-101.6 mg/L（r2=0.999 9），respectively.The limits of detection（LOD）were 0.25 mg/L and 0.05 mg/L，respectively.The RSDs of the method were 1.56%and 1.30%.The spiked recoveries of the two analytes were 98.86%-101.11%（RSD=1.02%）and 98.70%-101.19%（RSD=0.83%），respectively.
keywords: raw coffee bean chlorogenic acid caffeine high performance liquid chromatography（HPLC） content
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