Abstract:To study amino acid composition of protein hydrolysate of Oviductus Ranae （ORPH），to make a safety evaluation according to the acute toxicity and 30-days feeding test.RP-HPLC coupling with precolumn derivatization for determination of amino acid of ORPH.Acute oxicity test of ORPH was carried out by giving mice maximum tolerance dose，to observed the toxic reactions and mortality rate.In the 30-days feeding tset of ORPH，the high，middle，and low dose group was 100 times，75 times，and 50 times of the clinical dosage in human，respectively，then to observed the general behaviors，body weight，blood routine indiexes，serum biochemical indexes，organ weight，organ coefficient and tissue pathological change of mice.The results of amino acid composition showed that content of total essential amino acids accounted for 45.87%，content of total semi-essential amino acid accounted for 14.78%.The results of acute toxicity test showed the ORPH did not have toxicity，when the maximum dosage was 127 times of the clinical dosage in humans.Compared with control group，the 30-days feeding test showed that all detected indexes were no significant difference in each does group.In conclusion，ORPH has no acute toxic effect and is a safe function food on mice's growth，development，metabolism and viscera organs，meanwhile ORPH provides plenty of essential amino acids for organism.