Abstract:Phellinus igniarius was a very valuable function fungi in China，polysaccharides were the main bioactive components of Phellinus igniarius.In this experiment，the extractions of polysaccharide from fruiting body and two growth phase of mycelium were studied.The antioxidant activity was evaluated by 5 methods：total antioxidant capacity，scavenging ability of DPPH radical，scavenging effects on hydroxyl radicals，chelating ability to Fe2+，and scavenging ability of superoxide radical，the antioxidant experiments showed that the antioxidant effect of Phellinus igniarius polysaccharide was significantly higher than that of mycelium polysaccharide，different growth period mycelia polysaccharide had different antioxidant activity，polysaccharides within short growing period mycelium exhibited stronger antioxidant activity.The experimental results provided an important theoretical basis for industrial of Phellinus igniarius in the future.