Abstract:Determination method of zearalenone（ZEN）and ochratoxin A（OTA）in corn by high-performance liquid chromatography （HPLC）was established.Acetonitrile/water（90 ∶10，volume ratio）for the extraction solvent extracted zearalenone and ochratoxin A in corn，clean-up column purification；then the supernatant was dried by nitrogen，with the mobile phase to dissolve the residue，through the 0.22 μm organic filter to the inlet，HPLC detection.Methanol/water（60∶40，volume ratio）was used as mobile phase for ZEN，and acetonitrile/water/acetic acid（43 ∶56 ∶1，volume ratio）for OTA.The linearity range of ZEN and OTA were 0.5 μg/mL-6.0 μg/mL and 0.05 μg/mL-0.8 μg/mL，respectively，with correlation coefficient＞0.99.Recovery experiments were conducted by adding standard into the corn sample，with recoveries of 86.4%-114.1%，relative standard deviation of less than10%.The detection limit of ZEN and OTA were 5.0 μg/kg and 1.0 μg/kg，respectively.Different corn samples were found to be highly contaminated by ZEN and OTA using the method，with 71.4%of excessive rate.The method is simple and fast，good reproducibility，suitable for ZEN and OTA detection in the actual application.