Abstract:Macroporous adsorption resin was used to separate punicalagin from pomegranate husk.By comparing the adsorption rate and desorption rate，the macroporous adsorption resin was screened；by investigating gradient elution and isocratic elution on effect of separation and purification，separation mode was chosen；by using single-factor test，the parameters for separation confirmed.The result showed that the more punicalagin could be gotten by using LX-3010 resin in column of which the ratio of length to diameter was 5∶1 by isocratic elution.Single-factor test result showed the optimization condition for separation was：the content，the time，and the flow rate for adsorption were 2.0 mg/mL，30 min，and 1.5 mL/min；the content of ethnol，the time，and the flow rate for elution were 40%，180 min，and 1.5 mL/min，respectively.The punicalagin with purity of 80.12%was achieved under the condition.This processing could be a reference for comprehensive utilization of pomegranate husk.