Abstract:To study the effects of Pleurotus nebrodensis polysaccharide on the exercise capacity and anti-fa－ tigue of rats and the recovery of body movement after 12 hours of exhaustive exercise in rats. 100 male Wistar rats were divided into five groups: control group （A）， exhaustive group （B）， exhaustive resting group（C）， Pleurotus nebrodensis polysaccharide exhausted group （D）， white Pleurotus nebrodensis polysaccharide ex－ haustion resting group （E）. According to the test requirements， 5 groups of rats were subjected to 30 days of treadmill exercise. D and E were given Pleurotus nebrodensis polysaccharide every day. After the last exercise， the rats in group A were sacrificed and the related indexes were detected. The rats in group C and group E were sacrificed 12 hours after the operation， and the rats were sacrificed. The results showed that: GSH-Px activity， SOD activity and -SH content were significantly increased and the content of MDA decreased significantly after administration of Pleurotus nebrodensis polysaccharide. Indicating that Pleurotus nebrodensis polysaccharide can enhance the body's antioxidant capacity. After 12 h of rest， NOS and NO content were consistent with the pre-exercise indexes, which indicated that the polysaccharides of Pleurotus nebrodensis could enhance the re－ covery ability of the body and enhance the anti -fatigue ability. Pleurotus nebrodensis polysaccharide can strengthen the anti - fatigue and anti-oxidant function of rats， enhance the athletic ability and improve the re－ covery ability after exercise fatigue.