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食品研究与开发:2017,38(17):40-44
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核桃清蛋白抗氧化肽的制备及其活性研究
刘威1,2,闵伟红1,2,*,刘春雷1,2,曹柏营1,2,赵凡睿1,2
(1. 吉林农业大学 食品科学与工程学院,吉林 长春 130118;2. 小麦和玉米深加工国家工程实验室,吉林 长春 130118)
Preparation and Activity of Antioxidant Peptide from Walnut Albumin
LIU Wei1,2, MIN Wei-hong1,2,*, LIU Chun-lei1,2, CAO Bai-ying1,2, ZHAO Fan-rui1,2
(1. College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,Jilin,China; 2. National Engineering Laboratory on Wheat and Corn Further Processing,Changchun 130118,Jilin,China)
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投稿时间:2016-12-15    
中文摘要: 以核桃清蛋白为原料,用碱性蛋白酶进行酶解制备抗氧化肽,通过单因素与响应面试验,分析确定制备核桃 清蛋白抗氧化肽的最佳工艺条件为:pH9.5,温度 45 ℃,底物浓度 4 %,加酶量 8 000 U/g,酶解时间 2.5 h。对最优条件 下制备的抗氧化肽进行活性研究,测定其 Fe2+螯合能力及对羟基自由基、DPPH·、ABTS+·3 种自由基的清除能力,结果表 明核桃清蛋白抗氧化肽对几种自由基均有显著的清除作用和金属离子螯合能力,是一种活性的的天然抗氧化剂。
Abstract:Walnut albumin were used as raw material, walnut albumin isolated was hydrolyzed by Alcalase to prepare antioxidant peptide. Through single-factor experiment and response surface method, the optimal hy- drolysis conditions were determined as pH of 9.5, temperature of 45 ℃, substrate concentration of 4 %, Al- calase concentration of 8 000 U/g and hydrolysis time of 2.5 h. To assess the antioxidant potential, walnut albu- min antioxidant peptide was tested for its ferrous ions chelating capacity and scavenging capacities against hy- droxyl radical,DPPH·, ABTS+·. The experimental results showed that walnut albumin antioxidant peptide had significant scavenging effect against several kinds of free radicals, and ferrous ions chelating capacity, and it was a kind of natural antioxidants which had stronger antioxidant activities.
文章编号:201717010     中图分类号:    文献标志码:A
基金项目:国家“863”计划项目(2013AA102206)
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