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投稿时间：2016-11-16
投稿时间：2016-11-16
中文摘要: 采用磷酸缓冲液-乙酸乙酯混合提取、正己烷除脂、MCX 柱净化，采用液相色谱-质谱法（LC-MS/MS）建立了 同时测定猪肉中泰妙菌素及 8-α-羟基泰妙菌素残留量的分析方法。结果表明：在泰妙菌素和 8-α-羟基泰妙菌素 加标浓度 5.0 μg/kg～500.0 μg/kg 范围内，相关系数分别为 0.999 8 和 0.999 7，线性关系良好；检测限（LOD）分别为 1.5 μg/kg 和 3.0 μg/kg，定量限（LOQ）分别为 5.0 μg/kg 和 10.0 μg/kg；平均回收率为 83.4 %～97.6 %，相对标准偏差（RSD）为 2.4 %～5.3 %。
中文关键词: 泰妙菌素 8-α-羟基泰妙菌素 液相色谱-质谱法 猪肉
Abstract:Extracting by using phosphate buffer and ethyl acetate mixed， and degreasing by n-hexane， and pu－ rifying by MCX column， the method was established for determination of the content of pleuromulin and 8-αhydroxy-pleuromulin in pork by liquid chromatography-mass spectrometry （LC-MS/MS）. The results showed that the correlation coefficients of pleuromulin and 8-α-hydroxy-pleuromulin were 0.999 8 and 0.999 7 in spiked concentration from 5.0 μg/kg to 500.0 μg/kg， and they had good linear relationsh. The limit of detection（LOD） were 1.5 μg/kg and 3.0 μg/kg， and the limit of quantification （LOQ）were 5.0 μg/kg and 10.0 μg/kg； the average recovery rate were from 83.4 % to 97.6 %， and relative standard deviations（RSD）were from 2.4 % to 5.3 %.
文章编号：201716031 中图分类号： 文献标志码：A
基金项目:
| 作者 | 单位 |
| 范宏 | 承德护理职业学院，河北 承德 067000 |
| Author Name | Affiliation |
| FAN Hong | Chengde Nursing Career Academy，Chengde 067000，Hebei，China |
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