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食品研究与开发:2017,38(16):20-23
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柳花提取物总黄酮含量及抗氧化活性的研究
王苗苗,韩飞,李慕春*
(新疆维吾尔自治区分析测试研究院,新疆 乌鲁木齐 830011)
Comparison of Total Flavonoids and the Antioxidant Activity in Flowers of Willow with Different Solvents
WANG Miao-miao,HAN Fei,LI Mu-chun*
(Xinjiang Academy of Instrumental Analysis, Urumqi 830011, Xinjiang, China)
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投稿时间:2016-11-17    
中文摘要: 采用 70 %乙醇、石油醚、氯仿、乙酸乙酯、正丁醇和水 6 种不同极性的溶剂对柳花进行提取,对各溶剂提取物 的总黄酮含量进行测定, 同时以总抗氧化能力、DPPH 自由基清除率为指标, 研究所得 6 种柳花提取物的抗氧化能 力。结果表明,总黄酮含量由大到小依次为乙酸乙酯提取物(108.71 %)>70 %乙醇提取物(39.84 %)>正丁醇提取物(34.36 %)>氯仿提取物(23.39 %)>石油醚提取物(20.97 %)>水提取物(6.21 %);总抗氧化能力由强到弱依次为乙酸 乙酯提取物(16.9 U/mL)>正丁醇提取物(15.79 U/mL)>70 %乙醇提取物(14.92 U/mL)>水提取物(13.94 U/mL)>氯仿提 取物(6.04 U/mL)>石油醚提取物(4.56 U/mL),DPPH 自由基清除率由高到低依次为 70 %乙醇提取物(93.8 %)>乙酸 乙酯提取物(93.22 %)= 正丁醇提取物(93.22 %)>水提取物(89.75 %)>氯仿提取物(78.07 %)>石油醚提取物(58.3 %)。因 此,不同柳花提取物均具有抗氧化作用,且抗氧化能力与其提取溶剂和总黄酮含量有关。
中文关键词: 柳花  总黄酮  芦丁  分光光度法  抗氧化活性
Abstract:The components of flowers of willow were extracted by 70 % ethanol, petroleum ether, chloroform, ethylacetate , n-butyl alcohol and water ether with different polarity respectively. The research made items like the total flavonoid content ,the total antioxidant ability, DPPH·clearance rate , as indexes and evaluated the antioxidant ability of six kinds of extracts of flowers of Willow. The results showed that the order sequenced by the total flavonoid content from strong to weak was :ethyl acetate extract (108.71 %) > 70 % ethanol extract (39.84 %) > n-butyl alcohol extract (34.36 %) > chloroform extract (23.39 %) > petroleum ether extract (20.97 %) > water extract (6.21 %);and the order sequenced by the total antioxidant ability from strong to weak was : ethyl acetate extract (16.9 U/mL) > n-butyl alcohol extract (15.79 U/mL) > 70 % ethanol extract (14.92 U/mL) > water extract(13.94 U/mL) > chloroform extract(6.04 U/mL) > petroleum ether extract(4.56 U/mL),and the order sequenced by the DPPH·clearance rate from strong to weak was :70 % ethanol extract (93.8 %) > ethyl acetate extract(93.22 %) = n-butyl alcohol extract(93.22 %) > water extract(89.75 %) > chloroform extract(78.07 %) > petroleum ether extract(58.3 %).Therefore,the flowers of willow's extracts in different kinds of solvents all have antioxidant effects, and the antioxidant capacity is related to the extraction solvent and the total flavonoid content.
文章编号:201716005     中图分类号:    文献标志码:A
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