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食品研究与开发:2017,38(14):178-182
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增殖因子对嗜酸乳杆菌液态培养的影响
张超凤,李情敏,严美婷,杜霞, 陈卫平,张凤英*
(江西农业大学 食品科学与工程学院,江西 南昌 330045)
Effect of Proliferation Factor on the Liquid Culture of Lactobacillus acidophilus
ZHANG Chao-feng, LI Qing-min, YAN Mei-ting, DU Xia, CHEN Wei-ping, ZHANG Feng-ying*
(College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China)
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投稿时间:2016-09-20    
中文摘要: 对嗜酸乳杆菌培养基中的碳源和增殖因子进行优化,以增加菌体的数量。先从碎米粉、玉米、小麦等原料的 水解糖液中,选出最佳的碳源及最佳浓度。然后用土豆汁、番茄汁、麦芽汁、白萝卜汁、乳糖、大豆分离蛋白等 6 种物质 作为增殖因子,采用单因素试验,选出效果较好的增殖因子,继而采用正交试验以确定最佳的增殖因子组合,结果表 明:最佳碳源及浓度为 4 °Bx 碎米水解糖液;最佳增殖因子组合为:6 %麦芽汁,0.9 g/100 mL 乳糖,7 %白萝卜汁,在此 条件下液态发酵活菌数可达 6.2×109 cfu/mL。
Abstract:To increase the number of Lactobacillus acidophilus, the best carbon source and optimize the breed- ing factors in the culture medium was chosen. From hydrolysised the broken rice, corn and wheat into sugar, elect the best carbon source and the best concentration. Then put the solidify, tomato juice, malt juice, white turnip juice, lactose, soy protein isolated as multiplication factor of substances, adopt single factor experiment, choose the multiplication factor with better effect, and then adopt orthogonal experiment to determine the best combination of multiplication factor, the results showed that the best carbon source and concentration for 4 °Bx bro- ken rice hydrolyzed sugar liquid; best multiplication factor combination was: 6 % malt juice, 0.9 g/100 mL lac- tose, 7 % white turnip juice, the living bacterium number coule be up to 6.2 × 109 cfu/mL under this liquid fer- mentation condition.
文章编号:201714038     中图分类号:    文献标志码:A
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