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投稿时间：2017-02-10
投稿时间：2017-02-10
中文摘要: 建立一种超高效液相色谱-串联质谱法检测乳品饮料中四环素、土霉素、金霉素、强力霉素残留的分析方法。 样品前处理用乙腈沉淀蛋白后，采用 HLB 小柱进行净化和富集。采用 Waters Atalantis T3 色谱柱（2.1 mm×150 mm， 3 μm）分离，以 0.1 %甲酸-乙腈为流动相的梯度洗脱模式下，4 种四环素类抗生素在 1.0 ng/mL~200.0 ng/mL 浓度范围 内线性良好，相关系数 r2 为 0.998 5~0.999 2，检出限为 0.5 μg/kg～2.0 μg/kg，定量限为 1.5 μg/kg～6.0 μg/kg，加标回收率 达到 82.5 %~91.5 %。
中文关键词: 超高效液相色谱-串联质谱 乳品饮料 四环素类
Abstract:A ultra high performance liquid chromatography mass spectrometry analysis method was established for the residues determination of tetracycline， oxytetracycline， chlortetracycline and doxycycline in dairy bev－ erage. After the sample was treated with acetonitrile， it was concentrated and purified by HLB solid phase ex－ traction column. The separation of target compound was performed on a Waters Atalantis T3 chromatographic column（2.1 mm × 150 mm， 3 μm） using 0.1 % formic acid-acetonitrile as mobile phase under gradient elution mode. The linear range of 4 tetracycline antibiotics components was in the range of 1.0 ng/mL-200.0 ng/mL with a correlation coefficient of 0.998 5-0.999 2. The detection limit was 0.5 μg/kg-2.0 μg/kg and the quantitative limit was 1.5 μg/kg-6.0 μg/kg. The recovery rate was 82.5 %-91.5 %.
keywords: ultra high performance liquid chromatography mass（UPLC-MS/MS） dairy beverage tetracycline antibiotics
文章编号：201713039 中图分类号： 文献标志码：A
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