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投稿时间：2017-03-31
投稿时间：2017-03-31
中文摘要: 以猪皮为原料，使用酶法提取胶原蛋白，对提取条件和过程进行筛选和优化，并对胶原产物的结构性质进行 表征。结果表明，胶原蛋白产物的提取率为 65.33 %；纯度为 87.38 %；热变性温度在 118.79 ℃；紫外最大吸收波长为 203 nm；相对分子质量为 3.63×105 g/mol；红外光谱和圆二色谱结果显示，产物具有典型的胶原蛋白吸收峰，说明提取 出的胶原蛋白三螺旋结构保存完整； 十二烷基硫酸钠聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electrophoresis，SDS-PAGE）结果显示所得胶原产物主要由 3 种亚基组分组成。
Abstract:The research used pigskin as raw material， which was treated by enzymes， to extract collagen. To confirm and optimize the best extraction condition and process， the structural properties of collagen product were characterized. The experiment results showed that the extraction yield of collagen product was 65.33 % and the purity was 87.38 %. The thermal denaturation temperature was 118.79 ℃. The maximum UV absorption wavelength was 203 nm. The relative molecular mass was 3.63 ×105 g/mol. Infrared spectrum and round two chromatographic experiments showed that the product had a typical collagen absorption peak， indicating the three helical structure of collagen extracted was intact； SDS -PAGE gel electrophoresis test showed that the product was mainly composed of three sub components.
文章编号：201713010 中图分类号： 文献标志码：A
基金项目:江苏省产学研联合创新资金项目（BY2014023-17）
| Author Name | Affiliation |
| JI Qian， SU Dan-dan，YING Hui-yan， CHEN Jing-hua* | Laboratory of Pharmaceutics and Pharmaceutical Materials Science， Jiangnan University， Wuxi 214122， Jiangsu， China |
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