本文已被：浏览 2553次 下载 1026次
投稿时间：2016-12-01
投稿时间：2016-12-01
中文摘要: 试验以牛血清白蛋白（BSA）为有机干扰物质，大肠杆菌为灭菌对象，通过悬液定量杀菌试验和理化分析方法研 究有机干扰物质对超氧化电位水（NEOW）杀菌效果影响。试验结果显示：在不含有机干扰物时，NEOW 作用 30 s，能够将 菌落数对数值为 7.76 的大肠杆菌完全杀灭。用无菌水对 NEOW 稀释后，pH 和氧化还原电位（ORP）变化不明显，有效氯 浓度（ACC）随着稀释度的增加而降低，但稀释至 60 %时同样保持快速杀灭能力。在干扰物作用下，随着 BSA 浓度增加， NEOW 杀菌效果降低。但 NEOW 在低浓度 BSA 的干扰下，可以通过增加杀菌时间或者增加有效氯浓度达到杀菌效果； 在高浓度 BSA 的干扰下，需要对消毒环境进行预先清洗降低干扰物的量再进行消毒。
Abstract:Bovine serum albumin （BSA） was as organic interfering substances， Escherichia coli was for steril－ ization object in the laboratory. Suspension quantitative germicidal test and physicochemical analysis method were carried out to observe the influence of organic interfering substance on germicidal efficacy of neutral elec－ trolyzed oxidizing water （NEOW）.The results showed that when without organic interfering substances， the killing logartithm of Escherichia coli exposed to the NEOW for 30 s was 7.76. pH and oxidation reduction po－tential（ORP）changesd were not obvious after dilution of NEOW. Available chlori-ne concentration（ACC） de－ creased as dilution， however， the 60 % of dilution of NEOW maintained same germicidal efficacy. Germicidal efficacy of NEOW was significantly reduced as the BSA concentration increased. But germicidal efficacy of NE－ OW could be available by increasing time or ACC at low conce-ntrations of BSA， it need to be cleaned to re－ duce the amount of organic interfering substances and then disinfected at high concentrations of BSA.
keywords: organic interfering substance Escherichia coli neutral electrolyzed oxidizing water germicidal efficacy
文章编号：201712041 中图分类号： 文献标志码：A
基金项目:国家重点研发计划生物安全重点专项（2016YFC1201504）
引用文本：
津ICP备20000603号-1