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投稿时间：2016-09-22
投稿时间：2016-09-22
中文摘要: 建立黑果枸杞中花青素的高效液相色谱检测方法。采用酸化乙醇溶液超声提取黑果枸杞中的花青素，置于 沸水浴中将花青素水解为常见的花色素。选用高效液相色谱-紫外检测器、Agilent-ZORBAX SB-C18 色谱柱，以乙腈/0.1 %磷酸溶液为流动相进行梯度洗脱，在 525 nm 波长处对 6 种常见花色素（矢车菊色素、天竺葵色素、飞燕草色素、 芍药色素、牵牛花色素和锦葵色素）进行定量检测。结果表明，6 种花色素在浓度为 0.2 mg/L～100 mg/L 范围内，与响应 值呈线性关系，分别获得标准曲线方程及相关系数，r＞0.999；矢车菊色素、天竺葵色素、飞燕草色素、芍药色素、锦葵 色素的仪器检出限均为 0.02 mg/L， 牵牛花色素的仪器检出限为 0.05 mg/L；6 种花色素的平均回收率在 84 %～106 %， RSD 值在 0.5 %～7.9 %。
Abstract:A high performance liquid chromatographic （HPLC） method was estabolished for the determination of anthocyanidins in Lycium ruthenicum Murr.. The anthocyanidins in Lycium ruthenicum Murr. were extracted ultrasound with acidified ethanol， and then hydrolyzed into major anthocyanins in boiling water bath. A mixture of acetonitrile and 0.1 % phosphoric acid was used as the mobile phase gradient elution for the chromatographic separation on Agilent-ZORBAX SB-C18 column by HPLC-UV detector. The six kinds of anthocyanidins in－ cluding cyanidin， pelargonidin， delphinidin， peonidin， petunidin and malvidin were detected at 525 nm wave－ length. Results showed that the linearity of the method（r > 0.999） was good over the concentration range from 0.2 mg/L to 100 mg/L for six anthocyanidins. The limits of detection （LODs） of cyanidin， pelargonidinm， del－ phinidin， peonidin and malvidin were 0.02 mg/L，and the LOD of petunidin was 0.05 mg/L. The ranges of spiked average recoveries at different levels were 84 %-106 % with the relative standard deviations （RSDs） of 0.5 %-
7.9 % in a real sample.
文章编号：201712027 中图分类号： 文献标志码：A
基金项目:宁夏农林科学院院科技先导资金项目 (NKYQ-14-06)；农 业部农产品质量安全监管局特质性食用农产品营养组分识别与验证 评估项目（GJFP201601501）
| Author Name | Affiliation |
| ZHAO Zi-dan， GE Qian， NIU Yan， WANG Xiao-jing*， ZHANG Yan | Institute of Quality Standard and Testing Technology for Agro-Products of Ningxia， Yinchuan 750002， Ningxia， China |
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