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投稿时间：2017-03-19
投稿时间：2017-03-19
中文摘要: 利用喷雾干燥法可批量制备产业化、规模化用量的鲁氏酵母，显著缩短发酵周期，增加收益。本研究利用 YDP 培养基及 20 L 微生物发酵罐制备鲁氏酵母菌悬液，通过研究不同生长时间鲁氏酵母及培养基残糖含量、细胞干 重的变化确定最佳喷雾干燥时间。采用单因素及正交试验对保护剂及喷雾干燥条件进行优化。研究结果表明：在发酵 罐培养时间 27 h 时，细胞干重达到最大 18 g/L，此时为最佳喷雾干燥时间。保护剂最佳配比为脱脂奶粉 10 %、海藻糖
5 %、谷氨酸钠 1.5 %。确定最佳喷雾干燥条件为进口温度 115 ℃，进料流量 15 %（蠕动泵转速占比），保护剂与菌泥比 例 1 ∶ 1（g/L），按照上述条件得到发酵剂的存活率达到 83 %以上。
Abstract:The use of spray drying method can be batch preparation of industrial， large-scale amount of Zy－ gosaccharomyces rouxii， significantly shorten the fermentation cycle， increase revenue. The study was prepara－ tion of the suspension of Zygosaccharomyces rouxii on YDP and microbial fermentor. By studying the growth curve of Zygosaccharomyces rouxii yeast at different growth times and residual sugar content and cell dry weight and total number of viable bacteria. The single factor and orthogonal experiments were used to optimize the pro－ tective agent and spray drying conditions. Research indicates， culture time 27 h in microbial fermentor， cell dry weight was 18 g/L. The best proportion of protective agent were 10 percent of skim milk powder and five percent of trehalose and 1.5 % of sodium glutamate. Determine the best spray drying conditions were 115 ℃ of inlet tem－ perature and 15 % of feed flow and 1 ∶ 1 （g/L） ratio of preservative to bacteria， The survival rate of the starter was 83 % or more according to the above conditions.
文章编号：201712025 中图分类号： 文献标志码：A
基金项目:黑龙江八一农垦大学研究生创新科研项目（YJSCX2017-Y53）
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