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食品研究与开发:2017,38(12):83-87
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利用木瓜蛋白酶和α-淀粉酶澄清红茶茶汤的研究
钟艳梅,曾宪录,郑清梅,何曼怡
(嘉应学院 生命科学学院,广东 梅州 514015)
Research on the Clarification of Black Tea Soup with Papain and α-Amylase
ZHONG Yan-mei, ZENG Xian-lu, ZHENG Qing-mei, HE Man-yi
(School of Life Sciences, Jiaying University, Meizhou 514015, Guangdong, China)
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投稿时间:2016-09-23    
中文摘要: 为得到优质红茶饮料,利用外源木瓜蛋白酶和 α-淀粉酶对红茶茶汤进行沉淀澄清研究。结果表明:两种酶 均能显著提高茶汤透光率,起到澄清作用,且效果相当。木瓜蛋白酶最佳反应条件为酶添加量 0.3 U/mL、作用温度50 ℃、作用时间 45 min、pH6;α-淀粉酶最佳反应条件为酶添加量 30 U/mL、作用温度 45 ℃、作用时间 15 min、pH5,但 在各自最佳条件下两种酶未表现出协同作用。
中文关键词: 木瓜蛋白酶  α-淀粉酶  红茶  澄清
Abstract:The clarification effect of exogenous papain and α-amylase on black tea soup were investigated. Re- sults indicated that both two enzymes were able to significantly increase the transmittance rate, and the two en- zymes demonstrated no significant difference for clarification of tea. The optimal reaction conditions for papain were: addition amount of enzyme to be 0.3 U/mL, treatment temperature to be at 50 ℃, treatment time 45 min at pH 6;and the optimal conditions for α-amylase were: enzyme amount to be 30 U/mL, treatment temperature to be at 45 ℃, treatment time to be 15 min at pH 5.
文章编号:201712018     中图分类号:    文献标志码:A
基金项目:广东省教育厅自然科学基金(Yq2013152)
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