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食品研究与开发:2017,38(10):173-176
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鸡汤中一株耐热菌的分离与鉴定
骆琦,李娜,李楠,赵芳,刘常金*
(天津科技大学, 天津 300457)
Isolation and Identification of One Heat-resisting Strains in Chicken Soup
LUO Qi, LI Na, LI Nan, ZHAO Fang, LIU Chang-jin*
(Tianjin University of Science and Technology, Tianjin 300457, China)
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投稿时间:2016-08-18    
中文摘要: 实验室自制肉制品(鸡肉汤)中分离得到一株耐热菌,进行菌落形态、生理生化特征鉴定,并采用 Biolog 快速鉴 定结合 16S r DNA 序列分析两种方式鉴定,16S r DNA 序列分析结果表明此菌株属于芽孢杆菌属,Biolog 快速鉴定结果 显示此菌株属于芽孢杆菌属中的枯草芽孢杆菌。因此导致鸡汤高温加热后仍腐败变质的菌株为枯草芽孢杆菌。
中文关键词: 芽孢杆菌  16S r DNA鉴定  Biolog
Abstract:One heat-resisting strains , isolated by our lab-made meat product (chicken soup),was identi- fied by colony morphology, physiological and biochemical characteristics, 16S r DNA sequence analysis and Biolog Microstation System.The results from 16S r DNA sequence analysis shown that strains LQ-1 was belong to Bacillus genus.It indicated that it was belong to Bacillus subtilis spores with Biolog Microstation System. Therefore it is the Bacillus subtilis spores that resulted in the putrescence after chicken soup exposed to superheat for more than 10 minutes.
keywords: Bacillus  16S r DNA  Biolog
文章编号:201710039     中图分类号:    文献标志码:A
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