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投稿时间:2016-07-20
投稿时间:2016-07-20
中文摘要: 采用 Box-Behnken 试验设计,对超声提取昆布多酚工艺进行优化研究。最佳提取工艺是:乙醇浓度 30 %,液料 比 40 ∶ 1(mL/g),超声时间 32 min,超声温度 35 ℃,在此条件下昆布多酚的得率能够达到 4.485 mg/5 g。抑菌试验表明:昆 布多酚能够抑制大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌,对酵母的抑菌作用较小;对大肠杆菌和葡萄酒酵母的最低抑 菌浓度均为 5mg/mL,对枯草芽孢杆菌的最低抑菌浓度为 1.25mg/mL,对金黄色葡萄球菌的最低抑菌浓度为 2.5mg/mL。
Abstract:The Box-Behnken experimental design, optimized the ultrasonic extraction kelp polyphenols tech- nology research. The optimum extraction process were as follows: 30 % ethanol concentration, liquid to sol- id 40 ∶ 1 (mL/g), 32 min ultrasonic time, ultrasonic temperature 35 ℃, under the above optimum conditions, the extraction rate of kelp polyphenols reached up to 4.485 mg/5 g. Bacteriostatic experiment showed that: kelp polyphenols could inhibite Escherichia coli, Bacillus subtilis and Staphylococcus aureus, the bacteriostatic ef- fects of yeast was smaller; the minimum bacteriostasis concentration to E.coli and wine yeast were 5 mg/mL, the minimum bacteriostasis concentration to Bacillus subtilis was 1.25 mg/mL, the minimum bacteriostasis concen- tration to Staphylococcus aureus was 2.5 mg/mL.
keywords: kelp polyphenols ultrasonic assisted extraction the response surface optimization antibacterial activity
文章编号:201708011 中图分类号: 文献标志码:A
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