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投稿时间：2016-06-14
投稿时间：2016-06-14
中文摘要: 建立快速、准确测定乳饮料中纽甜含量的高效液相色谱检测方法。样品以 20 %乙腈水溶液为提取液，硫酸锌和 亚铁氰化钾为沉淀剂进行前处理，采用 ZORBAX SB-C18（250 mm×4.6 mm，5 μm）色谱柱，200 nm 检测波长，磷酸氢二铵 （0.020 mol/L，pH 3.5～4）：乙腈=（70 ∶ 30）为流动相进行等度洗脱分离。纽甜在 0.2 μg/mL～50.0 μg/mL 范围内线性良好，在0.8 mg/kg～5.0 mg/kg 加标范围内，回收率为 90.1 %～96.1 %，RSD 值为 1.7 %～2.1 %，最低定量限为 0.30 mg/kg。
Abstract:To establish an analysis method for the determination of neotame in milk drinks by High Performance Liquid Chromatography（HPLC）. Samples were extracted by 20 % acetonitrile water solution as extraction solu－ tion， zinc sulfate and potassium ferrocyanide as precipitating agent， and the purified solutions were analyzed by HPLC with a ZORBAX SB-C18 column （250 mm×4.6 mm，5 μm）using diammonium hydrogen phosphate （0.02 mol/L， pH 3.5-4）- acetonitrile solution（70 ∶ 30）as the mobile phase in equal degree program，the detec－ tion wavelength was 200 nm. The standard curves of neotame in the range of 0.2 μg/mL-50.0 μg/mL showed good linearity. The average recoveries of neotame was 90.1 %-96.1 % in range of 0.8 mg/kg-5.0 mg/kg addition， and the relative standard deviation was 1.7 %-2.1 %. The limits of quantitation for neotame was 0.30 mg/kg.
keywords: HPLC milk drinks neotame
文章编号：201705039 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| GUO Yue-ping， SHI Meng-di， XU Guang-wei， WEI He-wen， DONG Xiao-wei | Institute for Food and Drug Control of Jinhua City， Jinhua 321000， Zhejiang， China |
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