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投稿时间：2016-06-13
投稿时间：2016-06-13
中文摘要: 在单因素试验基础上应用正交试验方法对芒果核多酚提取条件进行优化并初步评价其体外抗氧化活性。试验 确定乙醇为最佳提取溶剂；各因素对多酚物质提取量的影响依次为料液比> 乙醇提取浓度=提取时间>提取温度；用乙 醇溶液提取芒果果核中多酚物质的最佳工艺条件为乙醇浓度 70 %，料液比 1 ∶ 25（g/mL），提取时间 120 min，提取温度60 ℃，芒果核多酚物质提取含量可达 4.36 mg/g。抗氧化活性试验结果表明芒果核多酚物质对羟基自由基、超氧阴离子自 由基及 DPPH 自由基的清除率分别为 90.9 %、83.3 %、90 %。优化的芒果核多酚提取工艺合理、可行，芒果核多酚物质具 有较强的抗氧化性。
Abstract:Based on the single factor experiments， orthogonal experimental design was employed to optimize the extraction conditions of polyphenol from mango kernel seeds and its antioxidative activity was evaluated. The re－ sults revealed the ethanol to be the best extraction solvent extraction and determined the factors affect the amount of time for the extraction as followed： ratio of raw material to water > concentration of ethanol = time> temperature. The optimum extraction condition were： concentration of ethanol 70 %， ratio of water to raw mate－ rial 1 ∶ 25（g/mL） ， extraction time 120 min， extraction temperature 60 ℃. Under these conditions， the extrac－ tion yield of polyphenol was 4.36 mg/g. The results of antioxidation experiments showed that the highest scav－ enging capacity of polyphenol against·OH， O2-·and DPPH free radical was 90.9 %， 83.3 % and 90 %， re－ spectively. The optimization of polyphenol extraction process technology was reasonable and feasible， polyphe－ nol from mango kernel seeds had stronger antioxidant activity.
文章编号：201705011 中图分类号： 文献标志码：A
基金项目:国家自然科学基金（21365011）；广西自然科学基金（2013 GXNSFAA019046）；贺州学院博士启动基金（HZUBS201515）
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