Abstract:The optimum conditions of purification of total flavones in Hibiscus manihot flower were investigated by using the macroporous adsorption resins. The the amount of total flavonoids by adsorption and desorption as an index，14 kinds of macroporous resins were screened by static adsorption and desorption experiments and then purification process parameters were optimized by dynamic adsorption and desorption tests. HZ801 resin was had the best adsorption and desorption effects. The optimal purification conditions are as follows：the sample concentration is 1 mg/mL ，the sample flow rate is 2 mL/min ， the sample size is 140 mL；the sampleis elutedby water with 2 BV and 70 % ethanol of 2.2 BV，the eluting velocityis 2 mL/min. In the optimal conditions， theaverage adsorption rate of total flavones in Hibiscus manihot flower was 95.03 %，and the purification factor is 4.04. HZ801 macroporous resin has the best effect on the enrichment of flavonoids，It is an ideal separation andpurification medium.