Abstract:Response surface methodology was employed to optimize the purification of flavonoids from the crude flavonoids extracts of sugarcane tips. The influence of three purification variables on flavonoids content was investigated ，including extraction pH value，extraction volume ratio and temperature. A central composite design was used to investigate the best conditions for purification of flavonoids produced by sugarcane tips，and a multivariable quadratic regression equation was developed. The results showed that the optimal purification conditions about flavonoids from the extracts of sugarcane tips were as follows：the sugarcane tip extractants were dissolved at 50 ℃in n-butanol，pH value = 3.27，extraction volume ratio was 3：1. Under above-mentioned con原ditions，the content of flavonoids was up to 166.4 mg/kg，which was similar to the predicted value.