Abstract:Using Escherichia coli，Bacillus subtilis，Staphyloccocus aureus，Bacillus cereus，Aerobacter aerogenes，Saccharomyces cerevisiae as tested microorganism to investigate the antimicrobial activity of F. velutipes extracts in vitro and each phase by determining the bacteriostatic circle and growth rate. The antimicrobial stability of F. velutipes extracts was also studied using Staphyloccocus aureus and Escherichia coli as the tested bacterial. The results revealed that F. velutipes extracts in vitro and each phase had strong antibacterial activities. Ethyl acetate phase showed significantly inhibit bacterial activity. Meanwhile，F. velutipes extracts in vitro and each phase had stronger bacteriostatic activity on gram-positive bacteria than gram-negative bacteria. The scope of Staphyloccocus aureus MIC was 3.25 mg/mL-12.5 mg/mL，MBC range from 3.25 mg/mL-12.5 mg/mL. The results showed that the extracts and each phase were stable to temperature，ultraviolet. F. velutipes had good antibacterial activities and antifungal activities. It can be used as a kind of healthy food（ drink）for furtherdevelopment and utilization.