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投稿时间：2015-09-25
投稿时间：2015-09-25
中文摘要: 以“卡拉克塞”伽师瓜为试验材料，采用甜瓜基因组辅助设计超氧化物歧化酶（Superoxide Dismutase，SOD）基因扩增引物。以伽师瓜cDNA为模板克隆超氧化物歧化酶基因CDS区序列，获得三条超氧化物歧化酶基因序列，分别命名为JSGSOD1（Genebank登录号KT160023）、JSGSOD2（Genebank登录号KT160024）和JSGSOD3（Genebank登录号KT160025），序列长度分别为987bp、804bp和459 bp。伽师瓜SOD与甜瓜SOD具有高度的同源性。JSGSOD1含CuSOD结构域，JSGSOD2含FeSOD家族结构域，而JSGSOD3含Cu/ZnSOD家族结构域。并利用相关软件对其理化性质分析和亚细胞器定位进行了预测分析。
Abstract:Melon genome was used to design superoxide dismutase（SOD）gene amplification primers.Three superoxide dismutase gene sequences obtained from“kalakesai”Jiashi melon cDNA were named as JSGSOD1 （Genebank accession number：KT160023），JSGSOD2（Genebank accession number：KT160024）and JSGSOD3 （Genebank accession number：KT160025），sequence lengths were 987 bp，804 bp and 459 bp，respectively. Jiashi melon SOD and melon SOD had a high degree of homology.JSGSOD1 contained CuSOD family domain，JSGSOD2 contained FeSOD family domain，JSGSOD3 contained Cu/ZnSOD family domain.The paper also analyzed its physical and chemical properties and subcellular localization analysis with relevant software.
keywords: Jiashi melon superoxide dismutase cloning analysis
文章编号：201616039 中图分类号： 文献标志码：
基金项目:国家自然基金资助项目（31460413）
| Author Name | Affiliation |
| HUANG Wei，FENG Zuo-shan，ZHANG Pei-ling，BAI Yu-jia | College of Food Science and Pharmacy，Xinjiang Agricultual University，Urumuqi 830052，Xinjiang，China |
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